Application of denaturing high - performance liquid chromatography ( DHPLC ) for monitoring 3 sulfate reducing bacteria in oilfields 4 5
نویسندگان
چکیده
Manuscript for Applied and Environmental Microbiology 1 2 Application of denaturing high-performance liquid chromatography (DHPLC) for monitoring 3 sulfate reducing bacteria in oilfields 4 5 Outi Priha, Mari Nyyssönen, Malin Bomberg, Arja Laitila, Jaakko Simell, Anu Kapanen and 6 Riikka Juvonen 7 8 VTT Technical Research Centre of Finland, Tietotie 2, Espoo, Finland 9 Kemira, Luoteisrinne 2, Espoo, Finland 10 Corresponding author: Outi Priha, [email protected] 11 12 Abstract 13 Sulfate reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment 14 and H2S-driven reservoir souring in oilfield sites. Successful management of industrial processes 15 requires methods that allow robust monitoring of microbial communities. This study investigated the 16 applicability of denaturing high-performance liquid chromatography (DHPLC) targeting the 17 dissimilatory sulfite reductase ß-subunit (dsrB) gene for monitoring SRB communities in oilfield 18 samples from the North Sea, USA and Brazil. Fifteen of the 28 screened samples gave a positive 19 result in real-time PCR assays, containing 9 x 10 6 x 10 dsrB gene copies ml. DHPLC and 20 denaturing gradient gel electrophoresis (DGGE) community profiles of the PCR positive samples 21 shared an overall similarity; both methods revealed the same samples to have the lowest and highest 22 diversity. The SRB communities were diverse, and different dsrB compositions were detected at 23 different geographical locations. The identified dsrB gene sequences belonged to several 24 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.01015-13 AEM Accepts, published online ahead of print on 21 June 2013
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